World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF PHYTOCHEMICALS AND ANTIBACTERIAL ACTIVITY OF MORINGA OLEIFERA AVAILABLE IN THE MARKET OF RAIPUR, CHHATTISGARH

Dr. Amit Dubey*, Wasim Raja and Bhavana Hirwani

ABSTRACT

The rate at which bacteria are resistant to the commercial antibiotics meant for their prevention and cure is of great concern. In view of this, many research groups are now engaged in medicinal plant research because natural products of higher plants may give a new source of antimicrobial agent. The antibacterial activity of Moringa oleifera (Lam.) Leaves extract belonging to the family Moringaceae, was determined using agar well diffusion method against some selected bacteria. Mueller Hinton Agar (MHA) (Becton Dicknson M. D USA), media was prepared according to the manufacturer's instruction. The present Study is the continuation of a program aimed at investigation of antimicrobial properties of Moringa oleifera leaf extract. The antibacterial activity was evaluated according to the disk diffusion method by using Gram positive; B. subtilis, S. aureus and Enterococcus and Gram negative; E. coli, Acetobacter, Citrobacter, bacteria. Concentrations of 25, 50, 75 and 100 mg/ml prepared from the dry leaves powder were used for antibacterial analysis using agar well diffusion methods. The methanol extracts of the plant leaves show an inhibitory effect on the growth of the tested bacteria. This Study shows that methenolic leaves extracts of Moringa oleifera Linn inhibit the growth of microorganism’s dose dependently. Phytochemical analyses of the leaf in solvents of varying polarity with methanol extract were also carried out. The phytochemical screening indicated the presence of flavonoids, tannins, steroid, alkaloid, saponins etc., in the extracts. The research showed that the higher reducing power of the methanolic extract could be due to the better solubility of the antioxidant components in water whereas the predominant antibacterial activity in organic solvent extracts, indicates that the active components responsible for the bactericidal activity are more soluble in organic solvents. These studies provide an evidence to support traditional medicinal uses of the plant.

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