World Journal of
Pharmaceutical and Life Sciences

PHYTOCHEMICAL SCREENING AND IN-VITRO EVALUATION FOR ANTIMICROBIAL ACTIVITY OF BAUHINIA PURPUREA L. ETAHNOLIC EXTRACT

Rohit M. Jamdar*, Kirti K. Jadhav, Megha S. Jadhav, Monika R. Jadhav, Mayur N. Ghotkar, Kaustubh U. Mane, Ganesh B. Vambhurkar and Shreenivas K. Mohite

ABSTRACT

Aim of this study was to evaluate antimicrobial activity and perform its phytochemical analysis and screening of the leaves of Bauhinia purpurea L .in the given study. Ethanolic extract of leaves of Bauhinia purpurea L. was tested against standard bacterial species - Staphylococcus aureus, Bacillus subtilis and fungal species Aspergillus niger, Trycophyton rubrum. In vitro antimicrobial test was performed by agar well diffusion method on Nutrient agar media and Sabouraud dextrose agar. Minimum Inhibitory Concentration (MIC) and relative percentage of inhibition (%) test was performed by Modified agar well diffusion method. Ethanol extract showed significantly inhibitory effect compared to marketed antibiotic formulation of Amikacin and marketed antifungal formulation of Fluconazol on tested organism and fungal species. Ethanol extract shows a broad spectrum of antimicrobial activity as it inhibited Gram positive bacteria (S.aureus and B.subtilis) and fungal species (A.niger, T.rubrum). With respect to phytochemical analysis and screening by using spectroscopic technique we easily evaluate Ethanolic extract characters.

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