World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF ACUTE TOXICITY AND IN VITRO ANTI-ANTHELMINTIC ACTIVITY OF ANTHRAQUINONES AND DERIVATIVES FROM PENTAS LANCEOLATA (FORSSK.) DEFLERS LEAVES

Dr. Pragati Kumar*, Ashish Kumar Sharma and Prabodh Shukla

ABSTRACT

Background: Anthelmintics are drugs that are used to treat infections with parasitic worms. This includes both flat worms, e.g., flukes and tapeworms and round worms, i.e., nematodes. These having great importance for human tropical medicine and for veterinary medicine. Objective: The objective of the present research work had been done to evaluate the toxicity and In vitro Anti-Anthelmintic potential of anthraquinones and derivatives isolated from Pentas lanceolata (Forssk.) Deflers leaves. Materials and Methods: For the study of toxicity, the OECD guidelines were used. Experimental animals (Swiss albino mice), Six mice in each, were grouped into six groups; five experimental groups and one negative control. In studying the acute toxicity, 100, 250, 500, 1000, 1500 and 2000 mg/kg isolated compounds was given via i.p. route of drug administration. For acute toxicity, a single dose was given, and gross behavioural changes were recorded. For anthelmintic test Swiss albino mice, male and females weighted in medium of 25-30gm and naturally infected for Syphucia obvelata and Aspiculuris tetraptera were selected and test compounds was dissolved in normal saline water and given orally to individual groups of mice. The eggs of parasites (Both species were determined separately) were count out on the first, second, third and fifth day. On the seventh day, one mouse from each group was sacrificed and the intestine was observed to on the subject of the presence of parasites. Results: The acute toxicity study resulted that all four selected test Compounds are comparatively safe when administered through i.p. rout to mice. Any kinds of toxic symptoms as well as mortality did not represent by the Compounds up to the dose of 250 mg/kg body weight. For Anti-Anthelmintic activity, all four isolated Test compounds and Piprazine citrate as standard drug was studied on the laboratory animals and the study concluded that, the groups of animals administered isolated Test compounds but not in control group showed a significant decreased in no. of eggs/gram of faeces excreted after 1st day of treatment. Results showed significant differences (P-value<0.001) in the number of eggs excreted by the S. Obvelata and A. Tetraptera and in mice, administering the test compounds and control group. Conclusion: From the studies, it was proved that Anthraquinones and derivatives isolated from leaves of Pentas lanceolata are an effective Anti-anthelmintic drug with the dose of 250 mg/kg.

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