World Journal of
Pharmaceutical and Life Sciences

ISOLATION AND DETERMINATION OF VITAMIN C (ASCORBIC ACID) IN DIFFERENT VARIETIES OF ROSES CULTIVATED IN QUETTA

Mashaal Sharif, Nizam Baloch*, Muhammad Asghar, Mohammad Faheem, Tamoor Khan, Murad Bibi, Muhammad Kamran Khan

ABSTRACT

Isolation and determination of vitamin C (ascorbic acid) in different varieties of roses (rose hip fruit) cultivated in the District Quetta, Balochistan is described for the first time in this document. Research and different studies show that the rose hip contain vitamin C as an active ingredient 500-1000%. Six samples of roses that belongs to family “Rosaceae” was first selected and then used for the determination of ascorbic acid. Vitamin C (Ascorbic acid) content in different varieties of roses (Rose hip fruit) was prepared and then has been determined by using spectrophotometric and titrimetric method. The kinetic reaction between methylene blue (MB+) and vitamin C was studied in the spectrophotometric method with the carried measurement at maximum absorption ?max. 665nm. It was founded that the vitamin C content in six different varieties were 2556, 7323, 9011, 2167, 5045 and 5234 mg/100 g of dry weight sample, whereas the titrimetric method shows the following results as 2405, 7406, 9261, 2007, 5104 and 5020 mg/ 100 g of dry weight of the six samples respectively. Obtained results shown that the investigated roses are good source of vitamin C.

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