World Journal of
Pharmaceutical and Life Sciences

ACUTE ORAL TOXICITY OF ANACID TABLET (POLYHERBAL FORMULATION) WITH ITS ANTI OXIDANT AND ULCER PROTECTIVE ACTIVITIES IN PYLORUS LEGATED INDUCED PEPTIC ULCER

Nilesh Patel, Dr. Janmejay Patel, Achal Patel, Dr. Ankitkumar M. Paneliya*

ABSTRACT

Introduction: Because of the large and growing use of natural derived substances in all over the world, it is not wise to rely only on tradition or millenarian beliefs; descriptive and pragmatic studies are useful and should be considered to attainment of reliable data regarding toxicity and efficacy of substance. Aim: To evaluate Anacid Tablet (AT) (polyherbal formulation) for its acute oral toxicity with antioxidant and ulcer protective activities in Peptic ulcer. Method: The certification of IAEC (SKPCPER/IAEC/2016-02/01) as per the CPCSEA was obtained for present study. The acute oral toxicity was done according to OECD guideline AOT-425 to know single dose (2000 mg/kg) toxicity of test drug on swiss albino mice. The effect of AT on peptic ulcer was assessed in Pylorus ligation induced ulcer model in Albino Wister Rats. Various gastric parameters (gastric volume, pH, free & total acidity, ulcer index) and oxidative stress marker level (superoxide dismutase-SOD, catalase, malondialdehyde-MDA) were assessed to know the effect of test drug in comparison with other controlled groups. Results: There were no any physical - behavioral changes and mortality were observed in any animal during period of 14 days. The obtained result of test drug treated group in comparison to various control and standard drug treated group shows significant increase in pH and decrease in gastric volume, total acidity, free acidity and ulcer index. Significant effect was observed on antioxidant marker level in test drug treated group as compare to other groups.Conclusion: The No-Observed-Adverse-Effect-Level (NOAEL) of Anacid Tablet is 2000 mg/kg as it did not produce any toxic effect at that dose. The Anacid Tablet has significant ulcer protective and antioxidant properties.

[Full Text Article]   [Download Certificate]