Abstract
MOLECULAR DIAGNOSIS OF CRYPTOSPORIDIUM PARVUM IN DIARRHEAL PATIENTS OF WASSIT PROVINCE
Dr. Abdulsadah A. Rahi* and Esraa Najim
ABSTRACT
Cryptosporidiosis is a disease caused by Cryptosporidium which is zoonotic protozoan parasite of the Apicomplexa phylum and the Cryptosporidiidae family. It is one of the most important pathogen in humans. The present study included the patients who were referred to Al Karamah Teaching Hospital and General Hospital of Martyr Fairuz at Wasit, Iraq during November 2015 to February 2016. A total of 100 children, aged from less than 1year to 11 years old, who suffered from watery diarrhea and abdominal pain were recruited. Stool samples were collected and examined using modified Zeihl-Neelson and Real-time PCR methods. The results showed that 29% of the samples were positive for C. parvum oocysts using modified Zeihl-Neelson and 26% of the samples were positive by Real-time PCR and 7 samples out of 71 samples negative in modified Zeihl-Neelson (7%), gave positive results by RT-PCR. The results showed that 33 samples out of 100 samples were gave positive by RT-PCR.
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