World Journal of
Pharmaceutical and Life Sciences

IDENTIFICATION OF ENTAMOEBA HISTOLYTICA IN DIARRHEAL PATIENTS USING REAL TIME PCR AT WASIT PROVINCE / IRAQ.

Dr. Abdulsadah A. Rahi* and Raheek Faris

ABSTRACT

Entamoeba hisolytica is a parasitic protozoan causing common diarrhea. The present study aimed to determine the prevalence of E. histolytica parasite from a total of 100 patients suffered from diarrhea attended to Al Al-Karamah Teaching Hospital and Al-Zahra'a Teaching Hospital at Wasit province / Iraq during the period from November 2015 to March 2016. Their ages were ranged from under 1 year – 60 years. Stool samples were collected and examined in direct wet smear method using normal saline, the results showed that 39 of samples were positive for E. histolytica. The age group (<1–10) years showed the highest rate of infection (63.88%), while patients aged (31 – 40) years showed the lowest rate infection (14.28%). The infection rate in male was higher (53.84%) than in female (46.16%). Results of E. histolytica were subjected to Real Time PCR appeared 35 samples out of 39 samples (35%), as well as 7 samples out of 61 (7%) samples negative in direct method, gave positive results. The results showed that 42 samples out of 100 samples were gave positive in Real Time PCR.

[Full Text Article]   [Download Certificate]