World Journal of
Pharmaceutical and Life Sciences

AMELIORATIVE ROLE OF MIXED HYDRO-METHANOL SOLVENT EXTRACT (60:40) OF ANDROGRAPHIS PANICULATA NEES ON CHROMIUM-INDUCED MEMBRANE DAMAGE AND ELECTRON TRANSPORT CHAIN COMPLEXES IN LIVER AND LUNGS

Durga Pada Dolai, Soumita Dey, Amit Nandi, Somenath Roy and Sankar Kumar Dey*

ABSTRACT

Mitochondria are the crossroads of several crucial cellular activities; they produce considerable quantities of superoxide radical and hydrogen peroxide, which can damage important macromolecules. Potassium dichromate (K2Cr2O7), a Cr (VI) compound, is the most toxic form of chromium and has been demonstrated to induce toxicity associated with oxidative stress in humans and animals. The aim of this study was to elucidate the ameliorative effects of mixed hydro-methanol solvent extract at the ratio of 60:40 of Andrographis paniculata Nees on chromium-induced membrane damage and electron transport chain (ETC) in liver and lungs mitochondria. In this investigation, a group of male Wistar rats (80-100 g) were induced by intraperitonial injection of vehicle (0.9% NaCl), K2Cr2O7 (0.8 mg / 100 g body weight / day), K2Cr2O7 plus mixed hydro-methanol solvent extract in the ratio of 60:40 at a dose of 500 mg/kg body weight daily at an interval of six hours after injection of K2Cr2O7 for a period of 28 days. Significantly decreased the Mito ETC Complex-I, II and III of Cr (VI) treated rats in liver and lungs mitochondria. On the other hand, increased the membrane cholesterol level but decreased the membrane phospholipid, membrane total ATPase and membrane Na+-K+ ATPase activity in response to Cr (VI) toxicity in liver and lungs. The results of the present study suggest that the administration of mixed hydro-methanol solvent extract in the ratio of 60:40 significantly supplement the chromium-induced such alterations in rat’s liver and lungs.

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