World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

ANTIFUNGAL ASPECTS OF BURKHOLDERIA SP.VIMP04 (JQ867373) INHIBITING SOIL-BORNE FUNGAL PATHOGEN OF SUGARCANE

Dr. Shrikumar Vijaykumar Mahamuni*

ABSTRACT

The objective of the present study was to investigate antifungal features of Burkholderia sp.VIMP04 (JQ867373) by dual culture and agar well diffusion methods against Ceratocystis paradoxa, the causative agent of pineapple disease of sugarcane. Culture filtrate and ethyl acetate extract obtained from culture supernatant of Burkholderia sp.VIMP04 (JQ867373) showed prominent antifungal activity. HPLC analysis confirmed that bacterium produced different organic acids including acetic, oxalic and formic acids. Acetic acid was predominantly produced by the bacterium. The GC-MS analysis of ethyl acetate extract revealed that antifungal fraction contained 09 principal compounds including tetratetracontane, 10-Heneicosene, eicosene 7-hexyl, cyclohexane (6-cyclopentyl-3-(3-cyclopentylpropyl) hexyl, heptadecane 9-hexyl and other fatty acid-, alcoholic- and phtalic acid derivatives. Hence the culture under study can be used as biocontrol agent.

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