Abstract
EFFECT OF PH AND NUTRITIONAL FACTORS ON BACTERIAL GROWTH AND CAROTENOIDS PIGMENTS PRODUCTION BY LOCAL BACTERIAL ISOLATES VIA VARYING ONE PARAMETER AT-A-TIME APPROACH
*Nor Aqeela M. N., Hairul Shahril M., Dr. Ismatul Nurul Asyikin I., Dr. Maryam M. R., Dr. Salina M. R. and Dr. Hanina M. N.
ABSTRACT
The local bacterial isolates, LO1 and LY1 were cultured in different pH media (pH 6, 7 and 8) and supplemented with 1% (w/w) of different nutritional factors, includes carbon sources (glucose, sucrose, starch), nitrogen sources (urea, casein, gelatine) and of inorganic salts (calcium chloride, sodium nitrate, sodium dihydrogen phosphate). It was found that the maximum orange pigment production was observed for LO1 local bacterial isolate cultivated in each modified LB media with pH 8, sucrose, gelatin and sodium dihydrogen phosphate respectively. Meanwhile, LY1 local bacterial isolate favoured to synthesis yellow pigment after being cultured separately in modified LB media with pH 8, glucose, casein and sodium dihydrogen phosphate.
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