World Journal of
Pharmaceutical and Life Sciences

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

PRODUCTION AND COMPARATIVE STUDY OF α- AMYLASE FROM WILD AND MUTANT HOT SPRING Bacillus subtilis

Dr. A. V. Girde*

ABSTRACT

?-amylase, a catalytic protein produced by moderately thermophilic bacteria, Bacillus subtilis was isolated from hot spring at Unkeshwar-[Dist.Nanded(M.S)]. Effect of various kinetic factors on amylase produced by wild and mutant Bacillus subtilis were studied and compared in the present study. pH and temperature optima for ?-amylase from isolated wild B. subtilis were found to be 8.5, and 65ºC respectively. The amylase from ultraviolet(UV) mutant and 5-Bromouracil(BU) mutant demonstrated higher activity at same pH whereas the optimum temperature was found to be raised by about 5 ?C to a temperature of 70?C for amylase from mutant as compared to amylase from wild B.subtilis , indicting its thermostable nature. Optimum time course of reaction and optimum substrate concentration for all three strains of B.subtilis ?-amylase was found to be 15 minutes and 4mg/ml respectively. This optimum time and concentration of substrate was found to be similar for amylase from mutant though with higher enzyme activity.

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