World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Urooj Hashmi* and Seema Firdouse


Objective: To separate, isolate and identify different phytochemical constituents present in ethanolic extract of leaves of Tarenna asiatica, a flowering shrub from Botanical garden near Himayatsagar road, Moinabad, Hyderabad. Methods: Initial phytochemical screening tests were performed to investigate the nature of the constituent’s present. Next step required the separation of individual components from the ethanolic leaf extract using HPTLC. Functional group identification was done via FTIR technique and 1H NMR was employed to elucidate the protonated compounds. Final identification and characterization of constituents was done using GC-MS analysis. Results: By phytochemical screening of the extract, chemical constituents recognised were alkaloids, carbohydrates, volatile oils, flavonoids, glycosides, tannins, steroids and triterpenoids. HPTLC helped in separation of the constituents and peaks obtained on FTIR spectrum lead to the determination of functional groups such as alkanes, alkenes, aromatic alkenes, halides, alcohols, Si-OR, S-OR, C-Cl, C-Br, S-S (disulphides). 1H NMR was employed to determine the nature of protons in the sample which in turn gives knowledge or an idea about the structural elucidation of components. GC-MS technique was employed for determination and identification of the phytoconstituents present in the ethanolic plant extract which were found to be as follows., benzaldehyde, glycerine, benzofuran-2,3-dihydro-, propane-1,3-diol 4-methyl-benzeneboronate, n-hexadecanoic acid, phytol, D-mannitol, propylene glycol monooleate and squalene. Conclusion: This development opens pathways for future research that can be undertaken to explore various other benefits and uses of Tarenna asiatica leaves in the field of medicine.

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