World Journal of
Pharmaceutical and Life Sciences

SIMULTANEOUS DETERMINATION OF HESPERIDIN METHYL CHALCONE, HESPERIDIN AND ASCORBIC ACID IN PHARMACEUTICAL DOSAGE FORM

Bhoomi B. Patel, Karan Mittal and Rajshree C. Mashru

ABSTRACT

Three precise, sensitive and selective UV Spectrophotometric methods were developed and validated for the simultaneous estimation of hesperidin methyl chalcone, hesperidin and ascorbic acid in pharmaceutical dosage form. Method A was Simultaneous estimation by solving matrix using Cramer's rule. In this method analytical wavelength selected for estimation of HMC, HP and AA were 348, 298 and 261 nm. The absorptivity values were calculated at each wavelength for each drug and amount of drugs in dosage form was calculated by solving the matrix using cramer's rule. Method B was first derivative zero crossing point. In this method analytical wavelength selected for estimation of HMC, HP and AA were 328.2 nm (ZCP for HP and AA), 340.2 nm (ZCP for HMC and AA) and 283.9 nm (ZCP for HMC and HP) respectively. Method C was double divisor ratio derivative. In this method spectra of ternary mixture was divided by standard spectrum of other two drugs. For estimation of HMC, spectra of ternary mixture was divided by 18 ?g/ml HP + 24 ?g/ml AA, for HP, spectra of ternary mixture was divided by 9 ?g/ml HMC + 36 ?g/ml AA and for estimation of AA ternary mixture spectra was divided by 6 ?g/ml HMC + 18 ?g/ml HP. All developed methods were validated according to ICH guidelines. Precision of all methods were calculated as intraday and interday precision and reported in terms of % RSD value, which was found to be less than 2% for all three proposed methods. Accuracy of all methods was reported in terms of % recovery, which was found to be in range of 95-105 %. All three developed methods were successfully applied for the simultaneous estimation of hesperidin methyl chalcone, hesperidin and ascorbic acid in pharmaceutical dosage form.

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