World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF METRONIDAZOLE, TETRACYCLINE, BISMUTH SUBCITRATE IN ITS BULK AND PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Shareefa Asra Shoeb* and Dr. R. Vani

ABSTRACT

A short selective, precise, accurate and sensitive for the estimation of Metronidazole, Tetracycline and Bismuth Subcitrate was done by RP-HPLC. The assay of Metronidazole, Tetracycline and Bismuth Subcitrate was performed with tablets and the % assay was found to be 100.99 and 100.70 and 100.50 which shows that the method is useful for routine analysis. The linearity of Metronidazole, Tetracycline and Bismuth Subcitrate was found to be linear with a correlation coefficient of 0.999 and 0.999 and 0.999 which shows that the method is capable of producing good sensitivity. The acceptance criteria of precision is RSD should be not more than 2.0% and the method show precision 0.6 and 0.5 and 0.7 for Metronidazole, Tetracycline and Bismuth Subcitrate which shows that the method is precise. The acceptance criteria of intermediate precision is RSD should be not more than 2.0% and the method show precision 0.7 and 0.4 and 0.3 for Metronidazole, Tetracycline and Bismuth Subcitrate which shows that the method is repeatable when performed in different days also.

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