World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

VALIDATION OF A FORCED DEGRADATION UPLC METHOD FOR ESTIMATION OF GLIBENCLAMIDE IN ORAL DOSAGE FORM

Dr. Osman Ahmed*, Mohd. Kareem Ahmed and Dr. Anas Rasheed

ABSTRACT

A selective, precise, accurate and stability indicating UPLC method is validated for estimation of Glibenclamide in oral dosage form. The method employed, with Hypersil C18 (100 mm x 2.1 mm, 1.7 ?m) column in gradient mode, with mobile phase of Methanol and Acetonitrile in the ratio of 80:20 %v/v. The flow rate was 1.2 ml/min and effluent was monitored at 272nm. Retention time was found to be 8.623±0.11 min. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) etc. in accordance with ICH guidelines. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 2- 10?g/ml respectively. The LOD and LOQ values for were found to be 0.19543(?g/ml) and 0.59223 (?g/ml) respectively. No chromatographic interference from excipients and degradants were found. The proposed method was successfully used for estimation of Glibenclamide in oral dosage form.

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