World Journal of
Pharmaceutical and Life Sciences

LYMPHOCYTES DNA DAMAGE FROM PANMASALA, GUTKHA, KHARRAH, CHEWING TOBACCO USERS AND CHAIN SMOKERS OF CENTRAL INDIA, BY USING SINGLE-CELL GEL ELECTROPHORESIS ASSAY

Fulse A. C. and Paikrao V. M.*

ABSTRACT

Background: Oral submucous fibrosis (OSMF) is a chronic, complex potential potent pre-cancerous condition characterized by juxta-epithelial inflammatory reaction and progressive fibrosis of the lamina propria and deeper connective tissues. As the disease progresses, the jaws become rigid to the point that the sufferer is unable to open his mouth. These events are further influenced by exposures to carcinogenic agents including panmasala, gutkha, kharrah and tobacco consumption. Single-cell gel electrophoresis assay or comet assay is a sensitive and rapid method for DNA strand breaks; it further provides information on amount of damage among individual cells. Aim and Objective: In this study, we aimed to analyse the lymphocyte DNA damage from panmasala, gutkha, kharrah and chewing tobacco users of central India, by using Single-cell gel electrophoresis assay. Materials & Methods: The peripheral blood samples from 60 addicted participants of age group 30-70yr were collected under sterile conditions in heparinised tubes used for Leukocytes culture and 30 healthy non-cancerous participants of same age group ware taken as control. The informed consent was obtained. The comet assay conducted using three well OxiSelect™ Comet Assay Kit and stained with vista green dye, the slides were analysed by using Olympus® BX 51 fluorescence microscope. The results were statistically analysed. Result: Mean age of addicted participants were 45.31± 16.24 (SD) and Mean age of control participants were 40.55± 11.15 (SD). Obtained comets were analysed by the CometScore 1.5 Software. The Comet score analysis shows that the mean % TDNA (Tail DNA) of comet in Leukocytes of addicted participants is found to be 32.61%±3.38(SD) than 7.16%±3.18(SD) mean % TDNA of control participants. Conclusion: It can be conclude that addiction to panmasala, gutkha, kharrah and chewing tobacco can damage DNA of peripheral blood leukocytes.

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