World Journal of
Pharmaceutical and Life Sciences

ISOLATION AND IDENTIFICATION OF NON-FERMENTING GRAM NEGATIVE BACILLI IN ALL CLINICAL SPECIMENS AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN IN TERTIARY CARE HOSPITAL OF CENTRAL INDIA

Bharat Singh, Anita Mutha and Dr. Manish Purohit*

ABSTRACT

Objective: This prospective observational study was done to identify the non-fermenting Gram negative bacilli (NFGNB) and performing their antimicrobial susceptibility pattern for commonly used antibiotics. Also to know the prevalence of different species of non-fermenting Gram negative bacilli in various clinical specimens. Materials and Methods: During period of one year (April 2017 to March 2018) all clinical specimens received in Microbiology Department of M.G.M. Medical College, Indore (M.P.) were processed according to the standard microbiological techniques. Result: 100 isolates of NFGNB were obtained by the processing of 1095 samples. The incidence of nonfermenting gram negative bacilli was 9.13%. Nonfermenters isolated predominatly were P.aeruginosa (78%) followed by Acinetobacter baumannii (19%) and Burkholderia cepacia complex (3%). NFGNB isolation was higher in male patients (54%) and most common in the age group of 60-70 years (18%). Majority of NFGNB were isolated from pus samples,(30%) followed by blood (20%). NFGNB had showed maximum susceptibility to meropenem (70%) followed by imipenem (67%) and amikacin (55%). Conclusion: Observations from the our study showed that the Different sensitivity pattern and multidrug resistance exhibited by nonfermenters pose a great problem in treating these infections and leads to high morbidity and mortality. These organisms have great potential to survive in hospital environment, so effective methods of sterilization and infection control measures should be implemented. Care in detection, evaluation of effective antibiotic options, judicious use of antibiotics by instituting antibiotic policy of combination therapy and rigorous infection control measures will help to fight against nonfermenters for effective management of patients. The wide spread variability of sensitivity profile of common hospital isolates, indicate that every hospital should monitor their antibiogram profile of these isolates from time to time to serve as a basis for empirical therapy in emergency situation.

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