EXPERIMENTAL AUTORADIOGRAPHY AUTORADIOGRAPHICAL STUDY OF THE REGENERATION OF DAMAGED CORNEA STROMA
Dr. Keto Davitaia*
Dr. Keto Davitaia*
The purpose of this study was to determine the source of the cells participating in regeneration of damaged corneal stroma by means of autoradiographic method. This process was investigated in white adult mice. The cornea of experimental animals was perforated up to lens by sterile preparative needle. For detection of proliferative activity of corneal stroma cells the pulse and late 3H-thymidine labeling method was used. 3H-thymidine (with specific activity of 52 cu/mM) with the dose of 2 ?cu per gram of animal weight was injected into the abdominal cavity of operated animals. Non-operated mice served as controls and they received 3H-thymidine injections according to the same protocol as the operated mice. In both cases material was fixed after 1,2,3,4,5,6,7,8,9,10 days. A great number of the new-formed stromal fibroblasts, which include 3H-thymidine just at the moment of cornea injury (late labeling) did not correspond to the low proliferative potential of stromal cells (pulse labeling) and could not be explained by their multiplication. Results of autoradiographic investigations have shown: labeled fibroblast-like cells are found only in a new-formed stroma, while stromal fibroblasts of intact cornea and stromal fibroblasts of intact parts of injured cornea are not labeled during the whole experiment, we can conclude that precursors of cells involved in regeneration of the cornea do not develop via multiplication of stromal cells of cornea; precursors of cells forming the infiltrate in the injured cornea multiply intensively beyond the borders of damaged area and then migrate into the inflammatory area.[Full Text Article]
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