World Journal of
Pharmaceutical and Life Sciences

VALIDATION OF A FORCED DEGRADATION UPLC METHOD FOR ESTIMATION OF FLUNIXIN

Syed Rashed Faizan Mehdi*, Syed Mohiuddin, Hussain Ali Khan and Syed Majid

ABSTRACT

A specific, precise, accurate and forced degradation UPLC method is validated for estimation of Flunixin. The method employed, with Hypersil BDS C18 (100 mm x 2.1 mm, 1.7 ?m) column in an gradient mode, with mobile phase of Methanol, 0.1% Orthophosphoric acid and Acetonitrile in the ratio of 45:35:20 %v/v/v. The flow rate was 0.1 ml/min and effluent was monitored at 239 nm. Retention time was found to be 3.453±0.08 min. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) etc. in accordance with ICH guidelines. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 1- 10 ?g/ml respectively. The LOD and LOQ values for were found to be 0.31607(?g/ml) and 0.95781(?g/ml) respectively. No chromatographic interference from parenteral’s excipients and degradants were found. The proposed method was successfully used for estimation of (parenterals form).

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