World Journal of
Pharmaceutical and Life Sciences

PHYSICOCHEMICAL EVALUATION, GC-MS AND ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF THE FRUIT PEELS OF CITRUS MACROPTERA MONTR. OF MEGHALAYA NORTH EAST INDIA

S. Bhutia*, T. Uriah, J. P. Mohanty, P. Pal and B. B. Kakoti

ABSTRACT

Day to day most of the bacteria became resistant towards antibiotics. The aim of our study is to evaluate the essential oil from the fruit peels of Citrus macroptera and to investigate the antibacterial property on antibiotic resistant bacteria. Physicochemical parameters like colour, solubility, refractive index, boiling point, specific gravity, carbon residue, iodine value, acid value, ester value were judged. The composition of the volatile oil was determined by GC-MS analysis. The antibacterial activity of the volatile oil was performed by agar well diffusion technique using antibiotic resistant bacteria. The oil was colourless, denser than water and insoluble in water but soluble in methanol, chloroform and diethyl ether. The pH was slightly acidic with refractive index and carbon residue were 1.463+0.0025 and 2.59+0.091 %. The major compounds found in the volatile oil were limonene, ?-myrcene, terpinene, cyclohexanol, ?- terpeneol, azulenemethanol, nootcatone, ?-copaene, caryophyllin, nonanal and careen. The oil showed more potency against gram positive bacteria like Staphylococcus aureus and Bacillus subtilis as compare to gram negative Salmonella typhi, Echerichia coli and Shigella dysenteriae. The volatile oil from the fruit peels of Citrus macroptera possesses good antibacterial property.

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