World Journal of
Pharmaceutical and Life Sciences

IDENTIFICATION AND IN VITRO ANTIMICROBIAL ACTIVITY OF MARINE STREPTOMYCES SPP. BACTERIA FROM TIGRIS RIVER SEDIMENTS IN BAGHDAD CITY

Talib Saleh Al-Rubaye, Mohsen Hashim Risan, Dr. Al-Rubaye Dalal* and Rabab O. Radi

ABSTRACT

The aim of this study is to isolate different Streptomyces spp. from Tigris river sediment soil samples and characterization of their bioactive compounds. About 44 (88%) samples of soil were suspected to contain Streptomyces. About 42 (84%) Streptomyces spp. have been isolated which showed different morphological characteristic. Primary screening for antimicrobial activities to these isolates against E. coli and S. aureus by cross streaking technique, indicated that 22 (52.38%) isolates have high antibacterial activities. Out of them 17 (40.47%) have antibacterial activities against S. aureus and E. coli, which obtained from the selected area (Abn 1, Abn 4, Abn 8, Abn 13, Abn 17, Abn 19, Abn 21, Abn 23, Abn 24, Jad 26, Jad 27, Jad 29, Jad 32, Kar 36, Kar 39, Kar 41, Kar 42, while only 5 (11.9%) isolates showed activities against S. aureus, from the selected area (Abn 3, Abn 7, Abn 11, Abn 16 and Jad 34). Secondary screening for bioactive compounds production by well diffusion method showed that 14 (63.63%) isolates have a very good zone of inhibition against S. aureus and E. coli. Samples with the highest antibacterial activities (21, M5, N- and D-) were chosen for optimization and characterization. The optimization of these isolates revealed that yeast extract and malt extract (ISP2) is more optimum than GYE with a highest OD (0.58) at day 4, at pH 7.8, NaCl tolerance 1% and temperature 30°C.

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