World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF TETRACYCLINE ANTIBIOTIC RESISTANCE: RELATION OF ANTIBIOTIC QUALITY PARAMETERS TO CASES OF RESISTANCE OCCURRING IN ARIS DISEASE IN TASIKMALAYA CITY HEALTH CENTER

Danni Ramdhani*, Sri Agung Fitri Kusuma, Resmi Mustarichie

ABSTRACT

Objective. Tetracycline is an antibiotic that usually used in treatment of acute respiratory infections (ARIs). One of the factors that support the occurrence of cases of antibiotic resistance is the antibiotic quality parameters of the potential and levels of antibiotics used. This study aims to determine the relationship between quality parameters of tetracycline antibiotics used in public health centers in Tasikmalaya city with cases of tetracycline resistance occurring in the area. Methods. This research method includes the determination of antibiotics potential with 3 + 3 pattern and the comparative value of the activity using agar diffusion method perforation techniques and antibiotic assay with UV-Visible spectrophotometry method with validation parameters: linearity, precision, accuracy, limit of detection (LOD) and limit of Quantification (LOQ). Results. The research shows the antibiotic potential of tetracycline is 101,71%. The validation method demonstrate that the method used fulfill the requirements of standard curve with concentrations in the range of 7.5-20 ?g/mL with a correlation coefficient of 0.9978, reproducibility 0.08%, average recovery percent of 99,97%, LOD 0,9961 ?g/mL, and LOQ 3.3203 ?g/mL. The Results of antibiotic assay is 102,76 %.

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