World Journal of
Pharmaceutical and Life Sciences

DETECTION OF COUNTERFEIT STEVIA PRODUCTS USING HPLC-ELSD

Supriyadi* and Meta Kartika Untari

ABSTRACT

Diabetes prevalence in Indonesia has been increasing every year. Indonesia becomes the world's 5th largest country with diabetic population, estimated to be about 10% of the population and about 51% of those were undiagnosed. People with diabetes mellitus are required to avoid sugar (sucrose) in their daily meals and replace it with safe sugar substitutes. Stevia from Stevia rebaudiana Bertoni is known as natural sugar substitutes which safe for people with diabetes melitus. Stevia rebaudiana is also traditionally used to treat diabetes mellitus. The standard mixtures of Stevioside, Rebaudioside A, Rebaudioside C and Dulcoside A were injected into HPLC-ELSD under optimum condition to obtain the chromatograms. Samples of stevia products were extracted with methanol, and methanol extract was injected into HPLC-ELSD under the same conditions as standard solution and chromatograms were obtained. Chromatograms from stevia product samples were compared with chromatograms from Stevioside standard solutions, Rebaudioside A, Rebaudioside C and Dulcoside A. Based on the results and the above discussion it can be concluded that from the five samples, samples 1 and 2 contain stevia sweetener, sample 3 contains no stevia sweetener but rebaudioside A. Sample 4 and 5 contain neither stevia nor steviol glycoside sweeteners.

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