World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Indian Science Publications, SOCOLAR, China, Cosmos Impact Factor, Research Bible, Fuchu, Tokyo. JAPAN, Scientific Indexing Services (SIS), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Scientific Indexing, UAE, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Indian citation Index (ICI), IFSIJ Measure of Journal Quality, International Scientific Indexing, UAE (ISI), Web of Science Group (Under Process), Directory of Research Journals Indexing, Scholar Article Journal Index (SAJI), International Scientific Indexing ( ISI ), 



Supriyadi* and Meta Kartika Untari


Diabetes prevalence in Indonesia has been increasing every year. Indonesia becomes the world's 5th largest country with diabetic population, estimated to be about 10% of the population and about 51% of those were undiagnosed. People with diabetes mellitus are required to avoid sugar (sucrose) in their daily meals and replace it with safe sugar substitutes. Stevia from Stevia rebaudiana Bertoni is known as natural sugar substitutes which safe for people with diabetes melitus. Stevia rebaudiana is also traditionally used to treat diabetes mellitus. The standard mixtures of Stevioside, Rebaudioside A, Rebaudioside C and Dulcoside A were injected into HPLC-ELSD under optimum condition to obtain the chromatograms. Samples of stevia products were extracted with methanol, and methanol extract was injected into HPLC-ELSD under the same conditions as standard solution and chromatograms were obtained. Chromatograms from stevia product samples were compared with chromatograms from Stevioside standard solutions, Rebaudioside A, Rebaudioside C and Dulcoside A. Based on the results and the above discussion it can be concluded that from the five samples, samples 1 and 2 contain stevia sweetener, sample 3 contains no stevia sweetener but rebaudioside A. Sample 4 and 5 contain neither stevia nor steviol glycoside sweeteners.

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