World Journal of
Pharmaceutical and Life Sciences

RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF CELECOXIB AND TRAMADOL HYDROCHLORIDE

Katravath Sony*, M. Venkatesh and A.Yasodha

ABSTRACT

An analytical simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Celecoxib and Tramadol HCL in bulk and marketed pharmaceutical dosage forms. This Separation was carried out on Symmetry C18 (250 x 4.6mm, 5μm particle size) column in isocratic mode with mobile phase containing Methanol and Phosphate Buffer were taken in proportion of 60:40% v/v adjusted to pH 3.6 using ortho phosphoric acid. The flow rate was 1.0 ml/min and effluent was monitored at 330 nm. The retention times for Celecoxib and Tramadol HCL were 2.131 and 3.056 min respectively. The method is useful in the quality control of bulk and pharmaceutical formulations. The method was validated for accuracy, precision, linearity, robustness, ruggedness and LOD & LOQ of standard solution. The developed method was found to be accurate, precise and selective for simultaneous determination of Celecoxib and Tramadol HCL in bulk and marketed pharmaceutical dosage forms.

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