World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

DEVELOPMENT AND VALIDATION OF UV-SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF DAIDZEIN IN SOY DRY EXTRACTS

Dr. Sachin Bhusari*, Akshay Lokhande and Pravin Wakte

ABSTRACT

A novel, simple, precise, accurate, and cost effective UV- visible spectrophotometric method for the estimation of daidzein in soy dry extracts according to the ICH Q2 (R1) guideline. Spiked daidzein solution was scanned over the UV-visible range for its wavelength of maximum absorbance. Various calibration standards of daidzein ranging from 0.5 to 8?g/ml were prepared. The calibration curve of concentration vs. absorbance was plotted. Various analytical method validation parameters viz. linearity, range, accuracy, precision, LOD, LOQ, and robustness, etc. were calculated. The wavelength of maximum absorbance of daidzein was found to be 248 nm. The correlation coefficient at the calibration range of 0.5-8 ?g/ml was found to be 0.999. The validation of the developed UV method was carried out by conducting linearity, accuracy, precision, robustness, ruggedness, limit of detection, and limit of quantitation studies. The developed UV method was found to be precise for the intra-day and inter-day study and shows percent relative standard deviation in the range of 0.54 to 1.52 & 0.67 to 1.69 respectively. The total percent recovery of daidzein was found to be 99.57 to 100.38 %. A simple, precise, and cost-effective UV- visible spectrometry method for the estimation of daidzein in a standardized dry extract of Soybean was developed. The said method was developed using a solvent containing an economical percentage of organic phase in aqueous media. Said validated UV- visible method can be efficiently used for the estimation of daidzein in soy dry extracts.

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