World Journal of
Pharmaceutical and Life Sciences

EFFECT OF VARIOUS TREATMENTS ON SEED GERMINATION IN M.EMARGINATA

Shweta Mathur* and Asha Goswami

ABSTRACT

Study aims various treatments on seeds of M.emarginata. Seeds treated with Hot water showed maximum imbibition (80%) but their germination was nearly inhibited (zero%) which might be due to membrane damage or death of embryo. Effect of GA3 (100 to 200 ppm), considered to be the replacement of chilling treatment, resulted in increase of germination percentage. Germination percentage showed increase from 100 ppm Gibbrellic Acid (GA3) and Benzyl Amino Purine (BAP) treated seeds to 150 ppm GA3 and BAP treated seeds. Maximum germination percentage (75.7% with GA3 and 70.7% with BAP) was observed in seeds treated with 150 ppm GA3 and BAP and imbibed for 96 hours. Higher concentration of GA3 and BAP (200 ppm) could not accelerate it further and infact decreased the rate of germination (46.6% and 41% respectively) with 96 hours imbibition. H2SO4 (40%) treatment could not prove to be highly successful (maximum 52.2% germination) and H2SO4 (80%) completely declined germination percentage (12.3% at 96 hours imbibition) due to blackening of seed coat. 40% and 80% HNO3 showed poor response(12.2% and 7.1% respectively) in comparison to H2 SO4 and normal water. Various effects like prosoaking, scarification, acid treatment, chilling, hot water treatment, growth regulators treatment are well known to induce germination in dormant seeds. ABA (Abscissic Acid) has been shown to be involved in regulating seed dormancy and GA3 (Gibberellic Acid) known to counteract the inhibitory effect of ABA .Growth hormones are the best activators to increase the rate of germination in M emarginata.

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