World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

DERIVATIVE ULTRA-VIOLET SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ARTEMETHER AND LUMEFANTRINE IN BULK AND FORMULATIONS

Dr. Prasanth S. S.* and Snija Rukku

ABSTRACT

A derivative Ultra Violet Spectrophotometric method was developed to estimate Artemether and Lumefantrine simultaneously in bulk as well as in formulations The method is based on comparing area of a first derivative curve of Artemether and Lumefantrine. The area between 215.80-245.0 nm for Artemether and area between 263.60- 283.40 nm for Lumefantrine was fixed as analytical wavelength for the determination in the first derivative mode (n=5) The calibration curve of both the drugs was plotted. The linear relationship between concentration and absorbance of both drugs were evaluated over the concentration range in1-9 ?g/ml and 6-54 ?g/ml for ART and LUM, respectively. The linearity ranges for ART and LUM were replicated for five times. The sensitivity of method was measured in terms of Limit of Detection (LOD) and Limit of Quantification (LOD). Intra-day precision was carried out by performing three replicates (at morning, afternoon and evening) of three different concentration (5, 7 and 9 ?g/ml for ART; 30, 42, 54 ?g/ml for LUM) on the same day and percent relative standard deviation (%RSD) was calculated. To ascertain the accuracy of proposed method, recovery studies were carried out by standard addition method by adding known amount of standard; 4, 5 and 6?g/ml for ART and 24,30 and 3 6 ?g/ml to the marketed tablet (5 ?g/ml ART and 30?g/ml LUM) at 80, 100 and 120 % level. The validated method was applied for the assay of commercial tablet of ATMITHER AL the results 97.1%±0.1 for ART and 95.5%±0.1 for LUM in combination.

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